Peripheral Blood Leukopaks®

Peripheral Blood Leukopaks

StemExpress' Peripheral Blood Leukopaks® provide enriched peripheral blood mononuclear cells (PBMCs) with low granulocyte and red blood cells. This highly enriched PBMC fraction requires minimal downstream processing (i.e. Ficoll) and can be used directly for isolating high yields of immune cells, such as CD4+ T cells, CD8+ T cells, CD19+ B cells, monocytes, and other immune cells. High cell yields allow researchers greater reproducibility of experiments from a single donor (minimizing donor-to-donor variation), a decrease in same cell variability, and scalability of experiments. In addition, up to 12 Leukopaks can be collected from the same donor in a given year, providing an ample amount of PBMCs for large-scale experiments and research studies.

High-Quality Leukopaks®

Cell-rich Leukopaks are extracted using an FDA-approved apheresis machine. Once collected, Leukopaks are sent out on the day of collection to ensure you receive a fresh product.

  • 1x10e10 TNCs/full bag
  • Highly-enriched PBMCs
  • Low granulocyte
  • Low hematocrit
  • 0.1-0.2% CD34+ cells
  • ≥90% viability (fresh)
  • HBV, HCV, HIV negative
  • IRB approved
Representative flow analysis of a peripheral blood leukopak after RCB lysis.

Customize Your Leukopak®

Our robust donor pool allows researchers the option to select specific donor criteria to suit their needs. Let us help you find the right donor.

  • 2500+ donors
  • Recallable donors
  • Donor demographics (i.e., age, weight, ethnicity, gender, blood type, etc.)
  • Donor lifestyle characteristics (i.e., BMI, smoker/non-smoker, etc.)
  • Medical history (i.e. allergies, medications, etc.)
  • HLA, RhD, and ABO typing available
  • Additional infectious disease screening (LCMV, etc.)
  • Much more…..

We Can Isolate the Cells for You

Increase reproducibility by letting our highly trained laboratory technicians isolate the primary cells from a Leukopak for you. We follow FDA CFR 21 Part 127 requirements of cGMP/cGTP regulations and employ FDA CFR 21 Part 11 data management software (ERP & QMS) that guarantees operational excellence and consistent quality products, ensuring confidence in your research.

NEW Frozen Leukopaks®

Our frozen Leukopaks provide convenience in the timing of your experiment without sacrificing viability.

Frequently Asked Questions

Can an apheresis donor be recalled for my experiments?

Yes, a donor is eligible to donate white blood cells every 4 weeks.

How many cells (PBMCs) can I isolate from a Leukopak®?

Usually around 8x10e9 PBMCs.

Is same day delivery available?

Yes, certain locations can receive same day courier delivery. We can also provide same day air shipments.

How to thaw a frozen Leukopak® for optimal viability?

Cells are very sensitive to many aspects of the thawing procedure, such as heat, DMSO, agitation, and high centripetal forces. Obtaining high viability after thawing a Leukopak can be difficult if not performed quickly and with good laboratory techniques. At StemExpress, our laboratory technicians have years of experience handling and thawing frozen cells and have designed a procedure that will help you obtain the highest viability of your cells. Click Leukopak Thawing Protocol to access the protocol.

Is LCMV testing an option?

Yes, LCMV testing can be requested on all our Leukopaks®.

Hi, have questions?

A StemExpress Expert is happy to help!

Call 530.303.3828 or Contact Us