Peripheral Blood CD4+CD25+ Regulatory T Cells, Frozen

$900
In stock
SKU
Category: Research Products
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PB425002C 2 million/vial $900 USD
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Product Description

Peripheral blood regulatory T cells (T-regs) are a subpopulation of T cells that play an important role in maintaining self-tolerance in the adaptive immune system by suppressing potentially deleterious activities of CD4+ helper T cells, which, if not regulated, can cause a variety of autoimmune disorders.

Human Peripheral Blood CD4+CD25+ Tregs are enriched in a multi-step process. First, peripheral blood mononuclear cells (PBMCs) are isolated by means of apheresis. Next, cells expressing CD8, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, and CD235a are depleted from the PBMC population using immunomagnetic particles leaving purified, untouched CD4+ T cells. Then, enriched CD4+ T cells are selected for CD25+ cells by positive selection using anti-CD25 immunomagnetic particles allowing for the enrichment of CD4+CD25+ regulatory T cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

More Information
Species Human
Disease State Healthy
Cell and Tissue Source Peripheral Blood
Product Type Primary Cells
Cell Type T Cells
Donor Attributes HIV-, HepB-, HepC-
Purity ≥85% by Flow Cytometry
Viability ≥80% by Flow Cytometry
Format Frozen
Contains CryoStor™ CS10 (10% DMSO)
Anticoagulant ACD-A

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet

 

Figure 1. Representative flow data of peripheral blood enriched CD4+CD25+ Tregs prior to cryopreservation.

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