Peripheral Blood CD19+CD27+ Memory B Cells, Frozen

$898
In stock
SKU
Category: Peripheral Blood
Select SKU Size / Day Price
PB192700.5C 0.5 million/vial $898 USD
PB192701C 1 million/vial $1,794 USD
PB192702C 2 million/vial $3,587 USD
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Product Description
Peripheral blood CD19+CD27+ memory B cells become active in the wake of a primary infection from a particular antigen. Memory B cells can persist for years or even a lifetime after an encounter with an antigen. The presence of these cells allows for a faster antibody response if the host comes into contact with the same pathogen.

Human CD19+CD27+ memory B cells are isolated from peripheral blood mononuclear cells. Cells expressing CD2, CD14, CD16, CD36, CD43, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving an enriched CD19+ fraction. CD27+ cells are then enriched from the CD19+ fraction using immunomagnetic anti-CD27 particles leaving purified CD19+CD27+ memory B cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
More Information
Species Human
Disease State Healthy
Cell and Tissue Source Peripheral Blood
Product Type Primary Cells
Cell Type B Cells
Donor Attributes HIV-, HepB-, HepC-
Purity ≥85% by Flow Cytometry
Viability ≥80% by Flow Cytometry
Format Frozen
Contains CryoStor™ CS10 (10% DMSO)
Anticoagulant ACD-A

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet

 

Figure 1. Representative flow data of peripheral blood enriched CD19+CD27+ memory B cells prior to cryopreservation.

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