Peripheral Blood CD14+ Monocytes, Frozen
Peripheral blood CD14+ monocytes originate in the bone marrow and move via the bloodstream to infected or damaged peripheral tissue. Once at the tissue, monocytes differentiate into specific tissue macrophage or dendritic cells. These cells are responsible for phagocytosis of foreign substances in the body and are capable of killing infected host cells via antibody-mediated cellular cytotoxicity.
Human peripheral blood CD14+ monocytes are isolated from the mononuclear cell fraction by means of negative selection. Cells expressing CD2, CD3, CD16, CD19, CD20, CD56, CD66b, CD123, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD14+ monocytes. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
|Cell and Tissue Source||Peripheral Blood|
|Product Type||Primary Cells|
|Donor Attributes||HIV-, HepB-, HepC-|
|Purity||≥90% by Flow Cytometry|
|Viability||≥80% by Flow Cytometry|
|Contains||CryoStor™ CS10 (10% DMSO)|
Product Information Sheet
Certificate of Analysis
Material Safety Data Sheet
Figure 1. Representative histogram of CD14+ monocytes from peripheral blood prior to cryopreservation.