Peripheral Blood CD14+ Monocytes, Frozen

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SKU
Category: Peripheral Blood
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PB14010C 10 million/vial $752 USD
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Product Description

Peripheral blood CD14+ monocytes originate in the bone marrow and move via the bloodstream to infected or damaged peripheral tissue. Once at the tissue, monocytes differentiate into specific tissue macrophage or dendritic cells. These cells are responsible for phagocytosis of foreign substances in the body and are capable of killing infected host cells via antibody-mediated cellular cytotoxicity.

Human peripheral blood CD14+ monocytes are isolated from the mononuclear cell fraction by means of negative selection. Cells expressing CD2, CD3, CD16, CD19, CD20, CD56, CD66b, CD123, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD14+ monocytes. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

More Information
Species Human
Disease State Healthy
Cell and Tissue Source Peripheral Blood
Product Type Primary Cells
Cell Type Monocytes
Donor Attributes HIV-, HepB-, HepC-
Purity ≥90% by Flow Cytometry
Viability ≥80% by Flow Cytometry
Format Frozen
Contains CryoStor™ CS10 (10% DMSO)
Anticoagulant ACD-A

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet

 

Figure 1. Representative histogram of CD14+ monocytes from peripheral blood prior to cryopreservation.

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