Peripheral Blood Mononuclear Cells, Fresh

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Category: Research Products
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Product Description
Mononuclear cells (MNCs) are composed of lymphocytes (T cells, B cells, and NK cells), monocytes, dendritic cells, and stem/progenitor cells. These cells are critical components of the immune system and play key roles in the humoral and cell-mediated immune responses. Mononuclear cells are commonly used in research and clinical applications in the areas of immunology, infectious disease, hematological malignancies, vaccine development, transplant therapy, and high-throughput screening.

Mononuclear cells are separated from peripheral blood by either a density gradient centrifugation protocol or by apheresis. MNCs are screened for the expression CD45 and checked for viability using flow cytometry. This highly pure population of cells makes them ideal for further isolations of specific cell types within the mononuclear fraction.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
More Information
Species Human
Disease State Healthy
Cell and Tissue Source Peripheral Blood
Product Type Primary Cells
Cell Type Mononuclear Cells
Donor Attributes HIV-, HepB-, HepC-
Viability ≥95% by Flow Cytometry
Format Fresh
Contains PBS with 5% FBS and 0.5% BSA
Anticoagulant ACD-A

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet

Publications

  • 1 Yang et al. (2016) Human Umbilical Cord-derived Mesenchymal Stem Cells Suppress Proliferation of PHA-activated Lymphocytes In Vitro by Inducing CD4+CD25highCD45RA+ Regulatory T Cell Production and Modulating Cytokine Secretion. Cell Immunol 302: 26-31. Abstract
  • 2 Yamada et al. (2014) G0S2 Inhibits the Proliferation of K562 Cells by Interacting with Nucleolin in the Cytosol. Leuk Res 38: 210-217. doi:10.1016/j.leukres.2013.10.006. Abstract
  • 3 Towobola et al. (2017) Novel Immunodeficient Rat Models Capable of Supporting the Growth of Human Tumor Xenografts. AACR 2017 Annual Meeting Poster. Poster
  • 4 Noto et al. (2018) The SRG™ Rat: A Novel SCID Rat for Humanization Studies. AACR 2018 Annual Meeting Poster. Poster
  • 5 Towobola et al. (2017) Novel Immunodeficient Rat Models Offers a Unique Platform for Drug Efficacy Studies in Human Tumor Xenografts. AACR-NCI-EORTC 2017 Annual Meeting Poster. Poster

 

Figure 1. Representative flow data of mononuclear cells enriched from peripheral blood.

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