Peripheral Blood Monocyte-Derived Immature Dendritic Cells, Fresh
Human peripheral blood monocyte-derived immature dendritic cells are derived from negatively selected monocytes. Non-monocytes are labeled and depleted from the peripheral blood mononuclear cell population using immunomagnetic particles leaving purified, untouched monocytes. Untouched monocytes are cultured for 4-5 days in culture medium containing 10% FBS plus GM-CSF and IL-4. After culture, cells are checked for the expression of CD11c, HLA-DR, and CD83 and the lack of CD14 expression by flow cytometry.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
|Cell and Tissue Source||Peripheral Blood|
|Cell Type||Dendritic Cells|
|Purity||≥90% CD11c and MHCII, <30% CD83, and ≤5% CD14 by Flow Cytometry|
|Viability||≥85% by Flow Cytometry|
|Contains||PBS with 5% FBS and 0.5% BSA|