Peripheral Blood CD8+ Cytotoxic T Cells, Frozen
Human peripheral blood CD8+ cytotoxic T cells, also known as cytotoxic T lymphocytes, defend the body against intracellular pathogens, such as viruses and bacteria, as well as cancerous cells. CD8+ T cells distinguish infected or diseased cells from healthy cells by recognizing antigens presented by MHC class I molecules located on the surface of the inflicted cells. Once the CD8 T cells recognize a specific antigen it releases cytotoxins that result in apoptosis of the infected or diseased cell.
Human CD8+ cytotoxic T cells are isolated from peripheral blood mononuclear cells by depleting cells expressing CD4, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, and CD235a using immunomagnetic particles leaving purified, untouched CD8+ cytotoxic T cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
|Cell and Tissue Source||Peripheral Blood|
|Cell Type||T Cells|
|Donor Attributes||HIV-, HepB-, HepC-|
|Purity||≥85% by Flow Cytometry|
|Viability||≥80% by Flow Cytometry|
|Contains||CryoStor™ CS10 (10% DMSO)|
Product Information Sheet
Certificate of Analysis
Material Safety Data Sheet
Figure 1. Representative histogram of peripheral blood enriched CD8+ cytotoxic T cells (93.72%).