Peripheral Blood CD4+CD45RA+ Naïve Helper T Cells, Frozen
Naïve helper T cells are mature T cells that have left the thymus and have yet to be exposed to an antigen presented by MHC class II molecules of an antigen-presenting cell (APC). In humans, CD4+ naïve helper T cells are constantly recirculating into the secondary lymphoid tissues in order to optimize the opportunity of finding their specific antigen.
Human peripheral blood CD4+CD45RA+ naïve helper T cells are enriched by depleting cells expressing CD8, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, CD45RO, TCRγ/δ, and CD235a from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD4+CD45RA+ naïve helper T cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
|Cell and Tissue Source||Peripheral Blood|
|Cell Type||T Cells|
|Donor Attributes||HIV-, HepB-, HepC-|
|Purity||≥90% by Flow Cytometry|
|Viability||≥80% by Flow Cytometry|
|Contains||CryoStor™ CS10 (10% DMSO)|
Product Information Sheet
Material Safety Data Sheet
Figure 1. Representative flow data of the enriched CD4+CD45RA+ cells (91.0%) from peripheral blood prior to cryopreservation.