Peripheral Blood CD4+CD25+ Regulatory T Cells, Fresh

$995
In stock
SKU
Category: Research Products
Select SKU Size / Day Price
PB425002F 2 million/vial $995 USD
-
+
Questions about shipping? See Shipping Info
Questions? Contact Sales:
530-303-3828
Contact Us
Product Description

Peripheral blood regulatory T cells (Tregs) are a subpopulation of T cells that play an important role in maintaining self-tolerance in the adaptive immune system by suppressing potentially deleterious activities of CD4+ helper T cells, which, if not regulated, can cause a variety of autoimmune disorders.

Human Peripheral Blood CD4+CD25+ Tregs are enriched in a multi-step process. First, peripheral blood mononuclear cells (PBMCs) are isolated by means of apheresis. Next, cells expressing CD8, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, and CD235a are depleted from the PBMC population using immunomagnetic particles leaving purified, untouched CD4+ T cells. Then, enriched CD4+ T cells are selected for CD25+ cells by positive selection using anti-CD25 immunomagnetic particles allowing for the enrichment of CD4+CD25+ regulatory T cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.


Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols
.

More Information
Species Human
Disease State Healthy
Cell and Tissue Source Peripheral Blood
Product Type Primary Cells
Cell Type T Cells
Donor Attributes HIV-, HepB-, HepC-
Purity ≥85% by Flow Cytometry
Viability ≥95% by Flow Cytometry
Format Fresh
Contains PBS with 5% FBS and 0.5% BSA
Anticoagulant ACD-A

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet

 

Figure 1. Representative flow data of peripheral blood enriched CD4+CD25+ Tregs prior to cryopreservation.

Hi, have questions?

A StemExpress Expert is happy to help!

Call 530.303.3828 or Contact Us