Peripheral Blood CD36+ Erythroid Progenitor Cells, Frozen
Human peripheral blood CD36+ erythroid progenitor cells are derived from cultured peripheral blood CD34+ cells. First, CD34+ cells are positively selected using immunomagnetic anti-CD34 microbeads from peripheral blood mononuclear cells. The isolated CD34+ cells are cultured in StemSpan™ SFEM, a serum-free expansion media for hematopoietic cells, supplemented with SCF, EPO and IL-3. Cultured cells are harvested after 10 days. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
|Cell and Tissue Source||Peripheral Blood|
|Cell Type||Stem/Progenitor Cells|
|Purity||≥85% by Flow Cytometry|
|Viability||≥70% by Flow Cytometry|
|Contains||StemSpan™ and 10% DMSO|