Peripheral Blood CD19+CD27- Naïve B Cells, Frozen | StemExpress

Peripheral Blood CD19+CD27- Naïve B Cells, Frozen

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Category: Research Products
Select SKU Size / Day Price
PB1927N00.5C 0.5 million/vial $690 USD
PB1927N01C 1 million/vial $1,311 USD
PB1927N02C 2 million/vial $2,634 USD
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Product Description
Peripheral blood CD19+CD27- naïve B cells are B cells that have not encountered an antigen. Upon antigen contact naïve B cells will undergo isotype class-switch recombination and somatic hypermutation and develop into plasma and memory B cells.

Human CD19+CD27– naïve B cells are isolated from peripheral blood mononuclear cells by means of negative selection. Cells expressing CD2, CD3, CD14, CD16, CD27, CD36, CD43, CD56, CD66b, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD19+CD27– naïve B cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
More Information
Species Human
Disease State Healthy
Cell and Tissue Source Peripheral Blood
Product Type Primary Cells
Cell Type B Cells
Donor Attributes HIV-, HepB-, HepC-
Purity ≥85% by Flow Cytometry
Viability ≥80% by Flow Cytometry
Format Frozen
Contains CryoStor™ CS10 (10% DMSO)
Anticoagulant ACD-A

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet


Figure 1. Representative flow data of peripheral blood enriched CD19+CD27- naive B cells prior to cryopreservation.

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