Peripheral Blood CD19+CD27+ Memory B Cells, Frozen
Human CD19+CD27+ memory B cells are isolated from peripheral blood mononuclear cells. Cells expressing CD2, CD14, CD16, CD36, CD43, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving an enriched CD19+ fraction. CD27+ cells are then enriched from the CD19+ fraction using immunomagnetic anti-CD27 particles leaving purified CD19+CD27+ memory B cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
|Cell and Tissue Source||Peripheral Blood|
|Cell Type||B Cells|
|Donor Attributes||HIV-, HepB-, HepC-|
|Purity||≥85% by Flow Cytometry|
|Viability||≥80% by Flow Cytometry|
|Contains||CryoStor™ CS10 (10% DMSO)|
Product Information Sheet
Certificate of Analysis
Material Safety Data Sheet
Figure 1. Representative flow data of peripheral blood enriched CD19+CD27+ memory B cells prior to cryopreservation.