Peripheral Blood CD19+ B Cells, Frozen

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Category: Research Products
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Positive Selection* Custom Inquire
PB19010C 10 million/vial $900 USD
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Product Description

Human B cells are lymphocytes that are characterized by the expression of CD19 on the cell surface. These cells are one of the main cell types involved in the humoral immune response. Upon activation, due to an encounter with an antigen, B cells will act as antigen-presenting cells (APCs), make antibodies against foreign antigens, and develop into memory B cells or plasma cells.

Human peripheral blood CD19+ B cells are isolated from the mononuclear cell fraction by means of negative selection. Cells expressing CD2, CD3, CD14, CD16, CD36, CD43, CD56, CD66b, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD19+ B cells. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.

*Our CD19+ B Cells are isolated by means of negative selection. However, we do offer the isolation of CD19+ B cells by positive selection as a custom order. Please inquire to obtain pricing for the positive selection of CD19+ B cells.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

More Information
Species Human
Disease State Healthy
Cell and Tissue Source Peripheral Blood
Product Type Primary Cells
Cell Type B Cells
Donor Attributes HIV-, HepB-, HepC-
Purity ≥90% by Flow Cytometry
Viability ≥80% by Flow Cytometry
Format Frozen
Contains CryoStor™ CS10 (10% DMSO)
Anticoagulant ACD-A

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet

 

Figure 1. Representative histogram of enriched CD19+ B cells from peripheral blood prior to cryopreservation.

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