Peripheral Blood Basophils, Frozen | StemExpress

Peripheral Blood Basophils, Frozen

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Category: Research Products
Select SKU Size / Day Price
PBBAS00.2C 0.2 million/vial $586 USD
PBBAS00.3C 0.3 million/vial $896 USD
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Product Description
Basophils are rare granulocytes that are most commonly found in peripheral blood and tissues. The primary function of basophils is to release histamine in response to allergens. Histamine causes blood vessels to dilate and increases blood flow to the inflicted area creating an inflammatory response. An increase in blood flow brings in a steady supply of white blood cells, such as eosinophils and neutrophils, to remove any foreign substance the immune system deems a threat. Basophils are also antigen-presenting cells (APCs) and promote the differentiation of naïve T cells into helper T cells.

Peripheral blood basophils are isolated from whole peripheral blood in a multi-step process. First, whole peripheral blood is treated with HetaSep to deplete red blood cells. Granulocytes are further enriched via density gradient centrifugation and processed to remove remaining red blood cells. Finally, basophils are negatively selected for by depleting cells expressing CD2, CD3, CD14, CD15, CD16, CD19, CD24, CD34, CD36, CD45RA, CD56, and CD235a from the granulocyte population using immunomagnetic particles leaving purified, untouched basophils. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
More Information
Species Human
Disease State Healthy
Cell and Tissue Source Peripheral Blood
Product Type Primary Cells
Cell Type Granulocytes
Donor Attributes HIV-, HepB-, HepC-
Purity ≥85% by Flow Cytometry
Viability ≥70% by Flow Cytometry
Format Frozen
Contains CryoStor™ CS10 (10% DMSO)
Anticoagulant CP2D

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet

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