Peripheral Blood Basophils, Frozen
Peripheral blood basophils are isolated from whole peripheral blood in a multi-step process. First, whole peripheral blood is treated with HetaSep to deplete red blood cells. Granulocytes are further enriched via density gradient centrifugation and processed to remove remaining red blood cells. Finally, basophils are negatively selected for by depleting cells expressing CD2, CD3, CD14, CD15, CD16, CD19, CD24, CD34, CD36, CD45RA, CD56, and CD235a from the granulocyte population using immunomagnetic particles leaving purified, untouched basophils. Isolated cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
|Cell and Tissue Source||Peripheral Blood|
|Product Type||Primary Cells|
|Donor Attributes||HIV-, HepB-, HepC-|
|Purity||≥85% by Flow Cytometry|
|Viability||≥70% by Flow Cytometry|
|Contains||CryoStor™ CS10 (10% DMSO)|