Peripheral Blood Basophils, Fresh
Basophils are rare granulocytes that are most commonly found in peripheral blood and tissues. The primary function of basophils is to release histamine in response to allergens. Histamine causes blood vessels to dilate and increases blood flow to the inflicted area creating an inflammatory response. An increase in blood flow brings in a steady supply of white blood cells, such as eosinophils and neutrophils, to remove any foreign substance the immune system deems a threat. Basophils are also antigen-presenting cells (APCs) and promote the differentiation of naïve T cells into helper T cells.
Peripheral blood basophils are isolated from whole peripheral blood in a multi-step process. First, whole peripheral blood is treated with HetaSep to deplete red blood cells. Granulocytes are further enriched via density gradient centrifugation and processed to remove remaining red blood cells. Finally, basophils are negatively selected for by depleting cells expressing CD2, CD3, CD14, CD15, CD16, CD19, CD24, CD34, CD36, CD45RA, CD56, and CD235a from the granulocyte population using immunomagnetic particles leaving purified, untouched basophils. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
|Cell and Tissue Source||Peripheral Blood|
|Product Type||Primary Cells|
|Donor Attributes||HIV-, HepB-, HepC-|
|Purity||≥85% by Flow Cytometry|
|Viability||≥85% by Flow Cytometry|
|Contains||PBS with 5% FBS and 0.5% BSA|