Mobilized Leukopaks, G-CSF, Fresh | StemExpress

Mobilized Leukopaks, G-CSF, Fresh

$12,000
In stock
SKU
Category: Research & Clinical Products
Select SKU Size / Day Price
MLE3GCSF4 Min x 3 Days - Apheresis Day 4 $12,000 USD
MLE4GCSF5 Min x 4 Days - Apheresis Day 5 $15,180 USD
MLE5GCSF6 Min x 5 Days - Apheresis Day 6 $15,840 USD
MLE3GCSF45 Min x 3 Days - Apheresis Day 4 & 5 $19,215 USD
MLE4GCSF56 Min x 4 Days - Apheresis Day 5 & 6 $21,630 USD
MLE5GCSF67 Min x 5 Days - Apheresis Day 6 & 7 $27,216 USD
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Product Description

Human mobilized peripheral blood Leukopaks provide a rich source of hematopoietic stem cells as compared to traditional Leukopaks. This allows researchers greater reproducibility of experiments from a single donor (minimize donor-to-donor variation), increase in scalability of experiments from a single product, and a decrease in same cell variability by obtaining high yields of specific cell types.

Mobilized Leukopaks are collected from healthy IRB consented donors that are injected with a combination of 10µg/kg/day of Granulocyte-Colony Stimulating Factor (G-CSF), which stimulates the bone marrow to produce hematopoietic stem cells and mobilizes them into the bloodstream, prior to the first day of collection. Mobilized mononuclear cells are collected by a trained technician using the Spectra Optia® Apheresis System one and two days post injection. After collection, mobilized Leukopaks are checked by flow analysis to verify the percentage of granulocytes, mononuclear cells, CD34+ cells, and cell viability.

• 2x10e10 TNCs/1 bag
• Enriched PBMCs
• Enriched CD34+ cells
• Low hematocrit
• Low granulocytes

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Custom Orders

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More Information
Species Human
Disease State Healthy
Cell and Tissue Source Peripheral Blood
Cell Type Mononuclear Cells
Donor Attributes Inquire
Viability ≥90% by Flow Cytometry
Format Fresh
Anticoagulant ACD-A
Size Minimum 20 billion TNCs/bag
Application Area Research
Injections 10μg/kg/day of G-CSF

Product Information Sheet

Certificate of Analysis

Publications

  • 1 Jang et al. (2020) Optimizing Lentiviral Vector Transduction of Hematopoietic Stem Cells for Gene Therapy. Gene Ther. Doi: 10.1038/s41434-020-0150-z. Abstract

 

Figure 1. Representative flow panel of fresh G-CSF mobilized Leukopak. Enriched PBMC population with low granulocytes (20.76%), high viability (97.65%), and a high percentage of CD34+ cells (1.68%).

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