Leukopaks are collected from healthy IRB consented donors, tested negative for HBV, HCV, and HIV, using the Spectra Optia® Apheresis System, where cell-rich mononuclear cells are extracted using a continuous flow system directly into a sterile collection bag containing the anticoagulant ACD-A. Up to 12 Leukopak collections can be performed on the same donor in a given year, providing an ample amount of PBMCs for large-scale experiments and research studies. Additionally, 200-300mL of plasma can be collected at the time of the donor draw.
The following are mean percentages of cell populations in a Leukopak:
• T cells - 55%
• Monocytes - 27%
• B cells - 9%
• NK cells - 8%
• CD34+ stem cells - 0.1%
• Granulocytes - 2.4%
We do not guarantee the percentage of cell populations in a Leukopak, as they can vary from donor to donor.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
We work closely with our customers to provide them with the product they need. For a custom order, please call and speak to one of our sales specialists at 530-303-3828.
|Cell and Tissue Source||Peripheral Blood|
|Cell Type||Mononuclear Cells|
|Viability||≥95% by Flow Cytometry|
Product Information Sheet
Certificate of Analysis
Material Safety Data Sheet
Figure 1. Representative flow analysis of a peripheral blood Leukopak after RBC lysis.