Leukopaks® are collected from healthy IRB consented donors, tested negative for HBV, HCV, and HIV, using the Spectra Optia® Apheresis System, where cell-rich mononuclear cells are extracted using a continuous flow system directly into a sterile collection bag containing the anticoagulant ACD-A. Up to 12 Leukopak collections can be performed on the same donor in a given year, providing an ample amount of PBMCs for large-scale experiments and research studies. Additionally, 200-300mL of plasma can be collected at the time of the donor draw.
The percentage of cells can vary from donor to donor. The following are average cell populations from a Leukopak®:
• T cells - 55% (range 45-60%)
• Monocytes - 27% (range 10-30%)
• B cells - 9% (5-15%)
• NK cells - 8% (range 5-10%)
• CD34+ stem cells - 0.1% (range 0.1-0.5%)
• Granulocytes - 2.3% (range 0.6-10%)
• Hematocrit - 3% (range 2-3.5%)
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
We work closely with our customers to provide them with the product they need. For a custom order, please call and speak to one of our sales specialists at 530-303-3828.
|Cell and Tissue Source||Peripheral Blood|
|Cell Type||Mononuclear Cells|
|Donor Attributes||HIV-, HepB-, HepC-|
|Viability||≥95% by Flow Cytometry|
Product Information Sheet
Certificate of Analysis
Material Safety Data Sheet
Figure 1. Representative flow analysis of a peripheral blood Leukopak® after RBC lysis.