Leukopaks are collected from healthy human donors, tested negative for HBV, HCV, and HIV, using the Spectra Optia® Apheresis System, where cell-rich mononuclear cells are extracted using a continuous flow method directly into a sterile collection bag containing the anticoagulant ACD-A. Leukopaks are quickly frozen in a medium containing the cryoprotective agent DMSO (10%) using a controlled rate freezer to ensure maximum viability. Up to 12 Leukopak collections can be performed on the same donor in a given year, providing an ample amount of PBMCs for large-scale experiments and research studies. Additionally, 200-300mL of plasma can be collected at the time of the donor draw.
• Enriched PBMCs
• 0.1-0.2% CD34+ cells
• Low hematocrit
• Low granulocytes
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
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|Cell and Tissue Source||Peripheral Blood|
|Cell Type||Mononuclear Cells|
|Donor Attributes||HIV-, HepB-, HepC-|
|Viability||≥80% by Flow Cytometry|