Peripheral Blood Monocyte-Derived Macrophages, Frozen | StemExpress

Peripheral Blood Monocyte-Derived Macrophages, Frozen

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Category: Research & Clinical Products
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PBMAC001.5C 1.5 million/vial $740 USD
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Product Description
Upon tissue damage or infection, monocytes leave the bloodstream and are rapidly recruited to the tissue, where they differentiate into tissue macrophages. Part of the innate immunity, macrophages are specialized cells that recognize, engulf and destroy target cells or pathogens. They are remarkably plastic and can change their functional phenotype depending on the various microbes or invaders they encounter. Along with their ability to clear pathogens, macrophages can also instruct other immune cells to take part in the immune response by acting as an antigen-presenting cell (APC).

The isolation of mature macrophages from tissue can be difficult, therefore the use of monocyte-derived macrophages (MDM) for functional studies is a very attractive alternative. Non-monocytes are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD14+ monocytes. The untouched monocytes are cultured for 4-5 days with 10% FBS in the presence of M-CSF and IL-4. After culture, cells are checked for the expression of CD11b, CD18, CD68, and HLA-DR by flow cytometry.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
More Information
Species Human
Disease State Healthy
Cell and Tissue Source Peripheral Blood
Cell Type Macrophage
Donor Attributes Inquire
Purity ≥ 90% MHC class II, CD11b, CD18, and CD68 by Flow Cytometry
Viability ≥80% by Flow Cytometry
Format Frozen
Contains CryoStor™ CS10 (10% DMSO)
Anticoagulant ACD-A
Application Area Research

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet


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