Peripheral Blood Leukopaks® | StemExpress

Peripheral Blood Leukopaks®

Peripheral Blood Leukopaks

StemExpress' Peripheral Blood Leukopaks® provide enriched peripheral blood mononuclear cells (PBMCs) with low granulocyte and red blood cells. This highly enriched PBMC fraction requires minimal downstream processing (i.e. Ficoll) and can be used directly for isolating high yields of immune cells, such as CD4+ T cells, CD8+ T cells, CD19+ B cells, monocytes, and other immune cells. High cell yields allow researchers greater reproducibility of experiments from a single donor (minimizing donor-to-donor variation), a decrease in same cell variability, and scalability of experiments. In addition, up to 12 Leukopaks® can be collected from the same donor in a given year, providing an ample amount of PBMCs for large-scale experiments and research studies.

Your Cells, Your Way!

HIGH-QUALITY LEUKOPAKS®

Learn more about our NEW Frozen Leukopak!

Cell-rich Leukopaks® are extracted using an FDA-approved apheresis machine. Once collected, fresh Leukopaks® are sent out on the same day to ensure a highly viable product or frozen immediately.

  • 1x10e10 TNCs/full bag
  • Highly-enriched PBMCs
  • Low granulocyte
  • Low hematocrit
  • 0.1-0.2% CD34+ cells
  • ≥95% viability (fresh)
  • HBV, HCV, HIV negative
  • IRB approved
Leukopak®

DONOR SELECTION

We provide customizable options to ensure you receive the Leukopak® you need when you need it.

  • Fresh or Frozen
  • Extensive donor pool
  • Recallable and repeat donors
  • Specific donor demographics (age, weight, ethnicity, etc.)
  • Donor lifestyle characteristics (BMI, smoker/non-smoker, etc.)
  • Medical history (allergies, medications, etc.)
  • HLA, RhD, and ABO typing available
  • Additional infectious disease screening (LCMV, etc.)
  • Much more....

CELL COLLECTION/PROCESSING

  • Special processing procedures available

EXPEDIENCY FROM ORDER TO DELIVERY

  • Custom media/buffer
  • Customizable packaging options
  • FedEx®, World Courier®, UPS®
  • Same day delivery in select cities

Popular Peripheral Blood Leukopak® Products

Frequently Asked Questions

Can an apheresis donor be recalled for my experiments?

Yes, a donor is eligible to donate white blood cells every 4 weeks.

How many cells (PBMCs) can I isolate from a Leukopak®?

Usually around 8x10e9 PBMCs from a Fresh Leukopak. However, this will be dependent on how you process the Leukopak (Ficoll, RBC lysis, etc.), all of which can decrease the amount of PBMCs.

Is same day delivery available?

Yes, certain locations can receive same day courier delivery on Fresh Leukopaks. We can also provide same day air shipments.

Is a Ficoll required to isolate PBMCs?

No, we offer a high-quality Leukopak® with minimal granulocytes and red blood cells. However, we do recommend an RBC lysis before isolating primary cells.

How do I thaw a frozen Leukopak®?

Cells are very sensitive to many aspects of the thawing procedure, such as heat, DMSO, agitation, and high centripetal forces. Obtaining high cell viability and recovery after thawing a Frozen Leukopak® can be difficult if not performed quickly and with good laboratory techniques. At StemExpress, our laboratory technicians have years of experience handling and thawing frozen human cells and have designed a procedure that will help you obtain the highest viability and recovery of your cells. Click Leukopak® Thawing Protocol to access the protocol.

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