Cord Blood CD8+ Cytotoxic T Cells, Fresh

Human CD8+ cytotoxic T cells, also known as cytotoxic T lymphocytes, defend the body against intracellular pathogens, such as viruses and bacteria, as well as cancerous cells. CD8+ T cells distinguish infected or diseased cells from healthy cells by recognizing antigens presented by MHC class I molecules located on the surface of the inflicted cells. Once the CD8+ T cells recognize a specific antigen it releases cytotoxins that result in apoptosis of the infected or diseased cell.
Human cord blood CD8+ cytotoxic T cells are enriched by depleting cells expressing CD4, CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, TCRγ/δ, and CD235a from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD8+ cytotoxic T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
Species | Human |
---|---|
Disease State | Healthy |
Cell and Tissue Source | Cord Blood |
Cell Type | T Cells |
Donor Attributes | HIV-, HepB-, HepC- |
Purity | ≥85% by Flow Cytometry |
Viability | ≥85% by Flow Cytometry |
Format | Fresh |
Contains | PBS with 5% FBS and 0.5% BSA |
Anticoagulant | CP2D |
Application Area | Research |
Product Information Sheet
Certificate of Analysis
Material Safety Data Sheet
Figure 1. Representative flow data of enriched CD8+ cytotoxic T cells from cord blood prior to cryopreservation.