Human CD8+ cytotoxic T cells, also known as cytotoxic T lymphocytes, defend the body against intracellular pathogens, such as viruses and bacteria, as well as cancerous cells. CD8+ T cells distinguish infected or diseased cells from healthy cells by recognizing antigens presented by MHC class I molecules located on the surface of the inflicted cells. Once the CD8+ T cells recognize a specific antigen it releases cytotoxins that result in apoptosis of the infected or diseased cell.

Human cord blood CD8+ cytotoxic T cells are enriched by depleting cells expressing CD4, CD14, CD15, CD16, CD19, CD34, CD36, CD56, CD61, CD123, TCRγ/δ, and CD235a from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD8+ cytotoxic T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.