Cord Blood CD34+ Stem/Progenitor Cells, Single Donor, Fresh

$1,050
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Category: Research Products
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Product Description

CD34 is a glycosylated transmembrane protein and represents a well-established marker for human hematopoietic stem and progenitor cells in peripheral blood, bone marrow, and cord blood. CD34+ cells are self-renewing, multipotent stem cells that give rise to all blood cells of the immune system through a process called hematopoiesis. As hematopoietic stem cells progress through hematopoiesis they generate the myeloid (monocytes, macrophages, granulocytes, megakaryocytes, dendritic cells, erythrocytes) and lymphoid (T cells, B cells, NK cells) lineages. The highly specialized cells that arise from hematopoietic stem cells work collaboratively in defending the body against infection and disease.

Human cord blood CD34+ stem and progenitor cells are positively selected using immunomagnetic anti-CD34 (clone QBEND10) microbeads from the mononuclear cell fraction. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.

Cord blood is collected from mothers that are negative for HIV, HepB, and HepC during pregnancy. Infectious disease testing, LCMV testing, and HLA typing on the cord blood can be provided as a custom order.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

More Information
Species Human
Disease State Healthy
Cell and Tissue Source Cord Blood
Product Type Primary Cells
Cell Type Stem/Progenitor Cells
Donor Attributes Maternal HIV-, HepB-, HepC-
Purity ≥90% by Flow Cytometry
Viability ≥85% by Flow Cytometry
Format Fresh
Contains StemSpan™
Anticoagulant CP2D

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet

Publications

  • 1 Halkias et al. (2015) Conserved and Divergent Aspects of Human T-cell Development and Migration in Humanized Mice. Immunol Cell Biol 1-11. doi:10.1038/icb.2015.38. Abstract
  • 2 Cleyrat et al. (2017) Gene Editing Rescue of a Novel MPL Mutant Associated with Congenital
    Amegakaryocytic Thrombocytopenia
    . Blood Advances 1: 1815-1826. doi.org/10.1182/bloodadvances.201600291.
    Abstract
  • 3 Takahashi et al. (2018) Enhanced Antibody Responses in a Novel NOG Transgenic Mouse with Restored Lymph Node Organogenesis. Front. Immunol 1(17). doi.org/10.3389/fimmu.2017.02017. Abstract

 

Figure 1. Representative flow data of the CD45 frequency, viability, and purity of enriched CD34+ cells from cord blood prior to cryopreservation.

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