Human T cells are lymphocytes that are generally characterized by the expression of CD3 on the cell surface. These cells originate in the bone marrow, migrate to the thymus to undergo maturation, and are one of the key cell types involved in cell-mediated immunity. T cells destroy invading organisms and aid in the activation of B cells through the thymus-dependent response. There are several different subsets of T cells, including helper T cells (naïve and memory), cytotoxic T cells (naïve and memory), regulatory T cells, and natural killer T (NKT) cells.

Human cord blood CD3+ pan T cells are isolated by means of negative selection. Cells expressing CD14, CD16, CD19, CD20, CD36, CD56, CD66b, CD123, and CD235a are depleted from the mononuclear cell population using immunomagnetic particles leaving purified, untouched CD3+ pan T cells. Isolated cells are characterized by flow cytometry to ensure a highly pure and viable cell population.

*Our CD3+ Pan T Cells are isolated by means of negative selection. However, we do offer the isolation of CD3+ T cells by positive selection as a custom order. Please inquire to obtain pricing for the positive selection of CD3+ T cells.

Cord blood is collected from mothers that are negative for HIV, HepB, and HepC during pregnancy. Testing on cord blood can be provided as a custom order.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.