Mononuclear cells (MNCs) are composed of lymphocytes (T cells, B cells, and NK cells), monocytes, dendritic cells, and stem/progenitor cells. These cells are critical components of the immune system and play key roles in the humoral and cell-mediated immune responses. Mononuclear cells are commonly used in research and clinical applications in the areas of immunology, infectious disease, hematological malignancies, vaccine development, transplant therapy, and high-throughput screening.

Mononuclear cells are separated from umbilical cord blood by means of a density gradient centrifugation protocol. CD133 depleted mononuclear cells are isolated from cord blood by removing the CD133+ population by immunomagnetic separation. The CD133 depleted mononuclear cells contain less than 0.1% CD133+ cells. The presence of CD133+ cells is assessed by flow cytometry prior to cryopreservation. The isolated MNCs can be used to isolate subpopulations or used as a control for donor matched CD133+ stem cell experiments (Please request the CD133 depleted flow-through fraction upon ordering CD133+ stem cells).

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.