Bone Marrow CD33+CD66+ Myeloid Cells, Frozen
$725
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Category: Research Products
Product Description
The antigens CD33 and CD66 are expressed on bone marrow derived myeloid cells such as myeloid progenitor cells, monocytes/macrophages and granulocytes (neutrophils, basophils, eosinophils). These cells play key roles in the adaptive and innate immune responses.
Bone marrow mononuclear cells (MNCs) are separated from whole bone marrow by a density gradient centrifugation protocol. Myeloid cells are obtained by positive selection using anti-CD33 and anti-CD66 antibodies and immunomagnetic separation techniques from the MNC population. Isolated cells are characterized by the expression of CD14 and CD66b from flow cytometry prior to cryopreservation.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
Bone marrow mononuclear cells (MNCs) are separated from whole bone marrow by a density gradient centrifugation protocol. Myeloid cells are obtained by positive selection using anti-CD33 and anti-CD66 antibodies and immunomagnetic separation techniques from the MNC population. Isolated cells are characterized by the expression of CD14 and CD66b from flow cytometry prior to cryopreservation.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
| Species | Human |
|---|---|
| Disease State | Healthy |
| Cell and Tissue Source | Bone Marrow |
| Product Type | Primary Cells |
| Cell Type | Myeloid Cells |
| Donor Attributes | HIV-, HepB-, HepC- |
| Purity | ≥90% by Flow Cytometry |
| Viability | ≥70% by Flow Cytometry |
| Format | Frozen |
| Contains | CryoStor™ CS10 (10% DMSO) |
| Anticoagulant | Na-Heparin |
Product Information Sheet
Certificate of Analysis
Material Safety Data Sheet
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