Cord Blood CD36+ Erythroid Progenitor Cells, Frozen

$673
In stock
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CB36001C 1 million/vial $673 USD
CB36002C 2 million/vial $1,344 USD
CB36002.5C 2.5 million/vial $1,680 USD
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Product Description
Cell differentiation along the erythroid lineage arises from hematopoietic stem cells within cord blood. The cell surface marker CD36, which is considered an early marker of erythrocyte differentiation, is acquired as the differentiation of this lineage progresses. However, the rarity and heterogeneity of the CD36 population makes it difficult to isolate in vivo. In order to obtain CD36+ erythroid progenitor cells, CD34+ hematopoietic stem cells (HSC) are isolated and cultured in serum-free expansion media supplemented with factors formulated to promote the expansion and differentiation of human erythroid progenitor cells.

Human cord blood CD36+ erythroid progenitor cells are derived from cultured cord blood CD34+ cells. First, CD34+ cells are positively selected using immunomagnetic anti-CD34 microbeads from the mononuclear cell fraction. The isolated CD34+ cells are cultured in StemSpan™ SFEM, a serum-free expansion media for hematopoietic cells, supplemented with SCF, EPO, and IL-3. Cultured cells are harvested after 10 days. Isolated cells are characterized by flow cytometry prior to cryopreservation to ensure a highly pure and viable cell population.

Cord blood is collected from mothers that are negative for HIV, HepB, and HepC during pregnancy. Testing on cord blood can be provided as a custom order.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
More Information
Species Human
Disease State Healthy
Cell and Tissue Source Cord Blood
Product Type Primary Cells
Cell Type Stem/Progenitor Cells
Donor Attributes Maternal HIV-, HepB-, HepC-
Purity ≥90% by Flow Cytometry
Viability ≥70% by Flow Cytometry
Format Frozen
Contains StemSpan™ and 10% DMSO
Anticoagulant CP2D

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