Clinical Grade Non-Transfusable Mobilized Peripheral Blood CD133+ Cells, G-CSF + Plerixafor, Frozen
Human CD133 is a transmembrane glycoprotein that is expressed on CD34+ and CD34– multipotent stem and progenitor cells that are involved in hematopoiesis. Unlike CD34+ cells, cord blood CD133+ cells are also expressed on circulating endothelial precursors that are involved in vasculogenesis. Additionally, cord blood CD133+ hematopoietic cells have been found to differentiate in vitro to cells of the ectodermal and mesodermal lineages implying that these cells have the ability to give rise to cells of non-hematopoietic lineages.
Human mobilized peripheral blood CD133+ stem and progenitor cells are isolated from the mononuclear cell fraction by means of positive selection. Cells expressing CD133 are enriched from the mononuclear cell population using CD133 immunomagnetic beads. CD133+ cells are characterized by flow cytometry before cryopreservation to ensure a highly pure and viable cell population.
Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols. All reagents and devices used in the collection and separation process are certified clinical grade. This item is for research use only.
|Cell and Tissue Source||Peripheral Blood|
|Product Type||Primary Cells|
|Cell Type||Stem/Progenitor Cells|
|Donor Attributes||HIV-, HepB-, HepC-|
|Purity||≥90% by Flow Cytometry|
|Viability||≥70% by Flow Cytometry|
|Contains||StemSpan™ and 10% DMSO|
|Injections||10μg/kg/day of G-CSF & 240μg/kg Plerixafor the day before collection|