Bone Marrow Mesenchymal Stem Cells, Frozen

$831
In stock
SKU
Category: Bone Marrow
Select SKU Size / Day Price
BMMSC001C 1 million/vial $831 USD
BMMSC002C 2 million/vial $2,300 USD
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Product Description
Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into the cells of the connective tissue in vitro, such as chondrocytes (cartilage), osteoblasts (bone) and adipocytes (fat). Although little is known of the phenotypic characteristics of MSCs in vivo, MSCs in culture are defined by the expression of CD73, CD90 and CD105 and the lack of CD11b, CD19, CD34, CD45 and HLA-DR. Cultured MSCs have shown promising applications in cell-based therapeutic strategies for specific tissues and cancer research.

Bone marrow mononuclear cells (MNCs) are separated from whole bone marrow by a density gradient centrifugation protocol. MNCs are cultured for 9-14 days using the MesenCult™-ACF Culture Kit. After culture, adherent cells are checked for >90% purity of CD73, CD90, and CD105 and <5% expression of CD14, CD34 and CD45 expression by flow cytometry before cryopreservation.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.
More Information
Species Human
Disease State Healthy
Cell and Tissue Source Bone Marrow
Product Type Primary Cells
Cell Type Mesenchymal Stem Cells
Donor Attributes HIV-, HepB-, HepC-
Purity ≥90% CD73, CD90, and CD105 by Flow Cytometry<5% CD14, CD34, and CD45 by Flow Cytometry
Viability ≥70% by Flow Cytometry
Format Frozen
Contains StemSpan™ and 10% DMSO
Anticoagulant Na-Heparin

Product Information Sheet

Certificate of Analysis

Material Safety Data Sheet

Figure 1. Representative flow data of enriched MSCs from bone marrow prior to cryopreservation. MSCs have a >90% expression of CD90, CD105, and CD73.

Figure 2. Representative flow data of enriched MSCs from bone marrow prior to cryopreservation. MSCs have <5% expression of CD14, CD34, and CD45 (panel of antibodies used).

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